A great horned owl found Monday after disappearing from its enclosure in Brandon during Tropical Storm Debby has died.

The owl, named Charlie, was supposed to receive a blood transfusion Thursday morning, but he died unexpectedly overnight, according to the Raptor Center of Tampa Bay.

Community members searched for the four-year-old ambassador owl for days after the center announced that Charlie had disappeared near Tampa Bay on August 5 during Tropical Storm Debby.

The Raptor Center posted several posts on social media asking the public for help in finding the bird. Community members knocked on about 500 doors and put up more than 2,000 flyers to help find Charlie, the Raptor Center said.

Photos of Charlie showed his majestic posture and puffy reddish-brown plumage. Perhaps most striking about the owl were Charlie’s yellow eyes – one clear, the other milky.

He was blind in one eye and unable to hunt on his own, said Nancy Murrah, president of the Raptor Center and Charlie’s caregiver.

A woman discovered Charlie in her backyard on Monday, not far from Murrah’s house. In a photo taken after Murrah found Charlie, the owl can be seen leaning against her chest.

But after days without food, Charlie had become thin. Although he ate something on Tuesday, he was too emaciated to survive, Murrah said in a phone call with the Tampa Bay Times on Wednesday.

She has been caring for the owl since she was six weeks old. Murrah said she was devastated.

Charlie was part of a family of great horned owls that was popular with bird watchers in Tampa Bay. Animal lovers found the owl lying on the ground one day when he was young, injured by a cat. Murrah took him in. After Charlie recovered, a woman gave him what she called Charlie’s “baby book.” The book contained photos she had taken of Charlie as a young owl and his family in the wild.

Charlie has since become involved in animal rescue and has often been shown to children. Even when he went missing, Charlie caught the attention of owls throughout Tampa Bay. Community members often called the Raptor Center, believing sick owls might be Charlie, which led to the rescue of several owls.